Journal
MACROMOLECULAR BIOSCIENCE
Volume 12, Issue 8, Pages 1034-1042Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201200043
Keywords
biological applications of polymers; biomaterials; biomimetic; hyaluronan; hydrogels
Funding
- NIH SBIR grant [1R43GM087765-01]
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These studies provide evidence for the ability of a commercially available, defined, hyaluronan-gelatin hydrogel, HyStem-C (TM), to maintain both mouse embryonic stem cells (mESCs) and human induced pluripotent stem cells (hiPSCs) in culture while retaining their growth and pluripotent characteristics. Growth curve and doubling time analysis show that mESCs and hiPSCs grow at similar rates on HyStem-C (TM) hydrogels and mouse embryonic fibroblasts and Matrigel (TM), respectively. Immunocytochemistry, flow cytometry, gene expression and karyotyping reveal that both human and murine pluripotent cells retain a high level of pluripotency on the hydrogels after multiple passages. The addition of fibronectin to HyStem-C (TM) enabled the attachment of hiPSCs in a xeno-free, fully defined medium.
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