4.7 Article

Multifunctionalized CMCht/PAMAM Dendrimer Nanoparticles Modulate the Cellular Uptake by Astrocytes and Oligodendrocytes in Primary Cultures of Glial Cells

Journal

MACROMOLECULAR BIOSCIENCE
Volume 12, Issue 5, Pages 591-597

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201100294

Keywords

CD11b antibody; dendrimers; glial cells; nanoparticles; targeted drug delivery systems

Funding

  1. Portuguese Foundation for Science and Technology [SFRH/BD/48406/2008, SFRH/BPD/63175/2009, PTDC/SAU-BMA/114059/2009]
  2. Foundation Calouste de Gulbenkian
  3. European FP7 Project Find and Bind [NMP4-SL-2009-229292]
  4. Fundação para a Ciência e a Tecnologia [SFRH/BD/48406/2008, PTDC/SAU-BMA/114059/2009] Funding Source: FCT

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The efficiency of the treatments involving CNS disorders is commonly diminished by the toxicity, reduced stability and lack of targeting of the administered neuroactive compounds. In this study, we have successfully multifunctionalized CMCht/PAMAM dendrimer nanoparticles by coupling the CD11b antibody and loading MP into the nanoparticles. The modification of the new antibody-conjugated nanoparticles was confirmed by S-TEM observation and 1H NMR and FTIR spectroscopy. Cytotoxicity assays revealed that the conjugates did not affect the viability of both primary cultures of glial and microglial cells. Trace analyses of FITC-labelled nanoparticles revealed that the uptake of antibody-conjugated nanoparticles was conserved in microglial cells but significantly decreased in astrocytes and oligodendrocytes. Thus, this study demonstrates that antibody conjugation contributes to a modulation of the internalization of these nanocarriers by different cell types, which might be of relevance for specific targeting of CNS cell populations.

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