Journal
MACROMOLECULAR BIOSCIENCE
Volume 12, Issue 7, Pages 937-945Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201100477
Keywords
cell sheet engineering; micropatterning; nano-grafting; thermoresponsive polymers; vascular smooth muscle cell phenotype
Funding
- Singapore Ministry of Education [M45120007]
- Nanyang Technological University
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A new method for ultrathin grafting of pNIPAm on PDMS surfaces is introduced that employs plasma activation of the surface followed by thermal polymerization. This method is optimized for human primary SMC attachment and subsequent intact cell sheet detachment by lowering the temperature. The contractile gene expression of the cells showed that the contractile phenotype of the SMCs which is induced by aligning the cells through micropatterning is more preserved after thermoresponsive cell sheet detachment in contrast with enzymatic detachment. Given its simplicity and low cost, this thermoresponsive grafting method can be utilized for engineering patterned cell sheets for future bottom-up tissue engineering techniques.
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