Journal
MACROMOLECULAR BIOSCIENCE
Volume 11, Issue 9, Pages 1201-1210Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201100041
Keywords
biological applications of polymers; functionalization of polymers; hydrogel coating; molecular recognition; orthogonal couplers
Funding
- DFG [EL 135/8-1, MO 682/8-1]
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The synthesis and characterization of a new difunctional coupler (4) based on trimethylolpropane (TMP) are described. The coupler is used to connect biologically active N-acetylglucos-amine (GlcNAc) on amino-reactive microtiter plates and on star-shaped poly[(ethylene oxide)-stat-(propylene oxide)] hydrogel layers in microtiter plates. The biological activity of immobilized carbohydrates is determined using an enzyme-linked lectin assay.
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