Journal
MACROMOLECULAR BIOSCIENCE
Volume 11, Issue 1, Pages 141-150Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201000280
Keywords
biomolecule immobilization; click chemistry; microspheres; poly(divinylbenzene); surface modification
Funding
- Istanbul Technical University (ITU)
- Ege University
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Hydrophobic-and/or hydrophilic-polymer-grafted PDVB microspheres are synthesized by the combination of hydrobromination and click-chemistry processes. The modified-PDVB microspheres and the intermediates at various stages of synthesis are characterized using GPC, H-1 NMR and FTIR spectroscopy and TGA analysis. Use of the microspheres as a support matrix for reversible protein immobilization via adsorption is investigated. The system parameters such as the adsorption conditions (i.e., enzyme concentration, medium pH) and desorption are studied and evaluated with regards to the biocatalytic activity and adsorption capacity.
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