Journal
MACROMOLECULAR BIOSCIENCE
Volume 10, Issue 10, Pages 1184-1193Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200900457
Keywords
enzymes; fluorescence; hydrogels; peptides; sensors
Funding
- EPSRC
- RSC
- BBSRC
- Wellcome Trust
- University of Manchester Strategic Fund
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The design of hydrogels that simultaneously report protease activity and remove excess protease from solution is elucidated. The hydrogels, based on amino-PEGA, combine enzyme-specific peptides flanked with FRET complimented by charged amino acid residues that facilitate protease uptake via short range electrostatic interactions. Enzymatic response was analysed using a combination of fluorescence spectroscopy, two-photon microscopy and UV/Vis spectroscopy. An optimised elastase-responsive hydrogel resulted in lowering of elastase levels below those typical of chronic wounds. The versatility of the modular-design approach was demonstrated by development of matrix metalloprotease and chymotrypsin sensitive systems.
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