Journal
MACROMOLECULAR BIOSCIENCE
Volume 10, Issue 4, Pages 384-392Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200900265
Keywords
biological applications of polymers; buffer capacity; pendant primary amine; poly(amidoamine); polyelectrolytes
Funding
- National Natural Science Foundation of China [20674058, 20874076]
- National Basic Research Program of China [2005CB623903, 2009CB930300]
- Ministry of Education of China [707043]
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Three poly(amidoamine)s with pendant primary amines were synthesized by Michael polyaddition of a diamine to N,N-methylenebis(acrylamide). The poly(amidoamine)s with primary and tertiary amines possess a high buffer capacity between pH = 5 and 7, which facilitates the escape of polymer/DNA complexes from endosomes according to the 'proton sponge hypothesis'. In vitro cytotoxicity of poly(amidoamine)s depends on the side-chain structure of the polymer. Polymers 1a-1c can condense plasmid DNA by electrostatic interactions to form nanosized polyelectrolyte complexes with a positive surface charge. In vitro transfection results indicate that the transfection efficiency of polymer 1b under optimized conditions is comparable to that of commercial branched PEI 25 000.
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