Journal
MACROMOLECULAR BIOSCIENCE
Volume 9, Issue 9, Pages 922-929Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200900005
Keywords
enzymes; immobilization of bioactive molecules; maleic anhydride copolymers; Subtilisin Carlsberg; thin films
Funding
- AMBIO project [NMP4-CT-2005-011827]
- 6th framework program of research and technological development of the European community
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Enzymes cleaving the biopolymer adhesives of fouling organisms are attracting attention for the prevention of biofouling. We report a versatile and robust method to con-fine the serine protease Subtilisin A (or Subtilisin Carlsberg) to surfaces to be protected against biofouling. The approach consists of the covalent immobilization of the protease onto maleic anhydride copolymer thin film coatings. High-swelling poly(ethylene-alt-maleic anhydride) (PEMA) copolymer layers permitted significantly higher enzyme loadings and activities than compact poly(octadecene-alt-maleic anhydride) (POMA) films. Substantial fractions of the immobilized, active enzyme layers were found to be conserved upon storage in deionized water for several hours. Ongoing studies explore the potentialities of the developed bioactive coatings to reduce the adhesion of various fouling organisms.
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