Journal
MACROMOLECULAR BIOSCIENCE
Volume 8, Issue 5, Pages 451-457Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200700220
Keywords
biofabrication; biotin; chitosan; electrodeposition; protein A; streptavidin
Funding
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1042870] Funding Source: National Science Foundation
- Directorate For Engineering
- Emerging Frontiers & Multidisciplinary Activities [1042881] Funding Source: National Science Foundation
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An alternative route to protein assembly at surfaces based on using the unique capabilities of biological materials for the spatially selective assembly of proteins is described. Specifically, the stimuli-responsive properties of aminopolysaccharide chitosan are combined with the molecular-re cognition capabilities of biotin-streptavidin binding. Biotinylated chitosan retains its stimuli-responsive properties and is capable of electrodepositing at specific electrode addresses. Once deposited, it is capable of binding streptavidin, which can mediate the subsequent assembly of biotinylated proteins. Spatially selective protein assembly using biotinylated Protein A and fluorescently-labeled antibodies is demonstrated.
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