Journal
MABS
Volume 2, Issue 5, Pages 480-499Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/mabs.2.5.12645
Keywords
monoclonal antibody; recovery; purification; chromatography; membrane; filtration; platform process
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Hundreds of therapeutic monoclonal antibodies (mAbs) are currently in development, and many companies have multiple antibodies in their pipelines. Current methodology used in recovery processes for these molecules are reviewed here. Basic unit operations such as harvest, Protein A affinity chromatography and additional polishing steps are surveyed. Alternative processes such as flocculation, precipitation and membrane chromatography are discussed. We also cover platform approaches to purification methods development, use of high throughput screening methods, and offer a view on future developments in purification methodology as applied to mAbs.
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