Probiotic potential of Lactobacillus strains with anti-allergic effects from kimchi for yogurt starters

Lactobacillus strains were isolated from kimchi and investigated for their potential use for probiotic yogurt starters. Two of the isolated strains have over 90% survival rate to artificial gastric acid (pH 2.5,1% pepsin) and artificial bile acid (0.3% oxgall). These strains were identified as Lactobacillus plantarum by 16S rRNA sequencing, and named as L plantarum SY11 and L plantarum SY12. The known carcinogenic enzyme, beta-glucuronidase was not produced by L plantarum SY11 and L plantarum SY12. These 2 strains were found to be resistant to several antibiotics and strongly adhered to intestinal cells. Antiallergic effect of L plantarum SY11 and L plantarum SY12 was demonstrated using nitric oxide (NO) and cytokine production. L plantarum SY11 and L plantarum SY12 were capable of significantly decreasing NO production, and reduced T helper 2-associated cytokines, cyclooxygenase-2, tumor necrosis factor-alpha, and inducible nitric oxide synthase compared to control. Yogurt samples produced using L. plantarum SY11 and L plantarum SY12 did not show significant differences in microbiological, physicochemical, and sensory properties compared with yogurt sample produced using commercial strain. Therefore, these two strains could be used as probiotic yogurt starters with antiallergic effects. (C) 2014 Elsevier Ltd. All rights reserved.