Antioxidant, antiacetylcholinesterase and antimicrobial activities of Cymbopogon schoenanthus L. Spreng (lemon grass) from Tunisia

Aqueous extract, proanthocyanidin rich extract, and organic extracts of Cymbopogon schoenanthus L Spreng (lemon grass) shoots from three different locations in South Tunisia were screened for their antioxidant, acetylcholinesterase and antimicrobial activities. In addition to the evaluation of these activities, the contents of flavonoids and total phenolic compounds were determined. Antioxidant activity measured by DPPH assay showed that the proanthocyanidin extract exhibited higher antioxidant activity than the aqueous extract. Extract concentration providing 50% inhibition (IC50) ranged from 16.4 +/- 6.8 mu g/mL to 26.4 +/- 6.8 mu g/mL. The antioxidant activity was also determined using the beta-carotene/linoleic acid bleaching test. The best results (IC50 = 0-11 +/- 0.10 mg/mL) were obtained with the proanthocyanidin extract of the plants collected from the desert region (Dhibat). The greatest acetylcholinesterase inhibitory activity (IC50 = 0.23 +/- 0.04 mg/mL) was exhibited by the ethyl acetate and methanol extracts of the plants collected from the mountainous region. It seems that extracts obtained with more polar solvents gave better results. The proanthocyanidin extracts showed a good antimicrobial activity against Streptococcus sobrinus at low concentration (MIC = 4 mg/mL). Therefore, these extracts could be used to prevent carious lesions by inhibiting S. sobrinus growth. (C) 2009 Elsevier Ltd. All rights reserved.