Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 43, Issue 1, Pages 39-44Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.lwt.2009.07.003
Keywords
Supercritical fluid extraction; Carotenoid; Composition; Pumpkin
Categories
Funding
- MOE/AAFC Program
- Guelph Food Research Center, Agriculture and Agri-Food Canada [S596]
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Pumpkin is a traditional food that is grown extensively worldwide and is believed to be beneficial to human health due to its high contents of carotenoids. The carotenoids in pumpkin were extracted by organic solvents and by supercritical carbon dioxide (SC-CO2), and then they were identified, quantified, and compared. beta-carotene (31 to 40 g per 100 g of total carotenoids) was the predominate carotenoid in pumpkin. Lutein and lycopene contents were much higher in SC-CO2 extracts than those in organic solvent extract. Cis-beta-carotene increased by more than two times in the SC-CO2 extracts, even at a relatively low temperature of 40 degrees C, over those in the solvent extracts, indicating both enhanced solubility and isomerization from trans- to cis-beta-carotene. The influences of modifier (10 mL/100 mL), temperature (40-70 degrees C), and pressure (25-35 MPa) Of SC-CO2 extraction on the change of carotenoid yields were also investigated. The highest yield (109.6 mu g/g) was obtained at 70 degrees C and 35 MPa, with a 73.7% recovery. Selective extraction could be achieved by adjusting the temperature and pressure. Higher proportions of all-trans-beta-carotene extracts were achieved at 40 degrees C under both 25 MPa and 35 MPa conditions. In order to extract more cis-isomers, a higher temperature of 70 degrees C was preferred. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.
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