Lipase production by solid fermentation of soybean meal with different supplements

The aim of this work was to study the production of extracellular lipases by solid state fermentation in soybean meal with different supplements. Lipase production by two microorganisms, screened by their potential for lipase production, was followed in terms of hydrolytic activity at different pH values(4, 7 and 9). The supplementation of the medium with urea and soybean oil significantly increased the enzyme production for all studied pH and microorganisms. Microorganism Penicillium P58 and P74 showed the possibility of production of different lipases with alkaline and acidic characteristics. In soybean meal supplemented with urea and soybean oil, this microorganism yielded 139.2 and 140.7U lipase/g of dry substrate in 48 h of fermentation, in alkaline and acidic conditions, respectively. Different behavior was observed when the enzyme extract was evaluated in neutral conditions, which yielded 180.0U lipase/g in 72 h. A new promising lipase producer strain was testedon soybean meal with different supplements. The strain produced a lipase with high activities by solid state fermentation of soybean meal supplemented with urea and soybean oil. (C) 2010 Elsevier Ltd. All rights reserved.