Significance of glomerular activation of the alternative pathway and lectin pathway in lupus nephritis

The objective of the present study was to elucidate the association between glomerular complement depositions belonging to the alternative (AP) and lectin (LP) pathways, and clinical findings of lupus nephritis (LN). Immunofluorescence (IF) was performed on 17 LN patients using antibodies against factor B, factor H, properdin, mannose-binding lectin (MBL) and L-ficolin. Compared with factor B/factor H negative patients (n = 9), positive patients (n = 8) showed longer duration of LN (p< 0.05) and more severe interstitial fibrosis (p< 0.05). Eleven patients had properdin deposition in glomeruli, and in three of them, with a duration of LN of less than 1month, factor B was undetectable. Compared with properdin negative patients (n = 6), positive patients (n =11) showed significantly higher urinary protein excretion (p< 0.01). MBL/L-ficolin positive patients (n = 11) also had significantly higher urinary protein excretion (p< 0.05) compared with negative patients (n = 6). An independent association was found between glomerular deposition of properdin and that of MBL/L-ficolin (p< 0.01) in addition to factor B/factor H. Traces of glomerular activation of AP and LP reflected the clinical status of LN. It appears that glomerular deposition of each complement component, especially properdin, may be an index of the histological activity of LN. Lupus (2011) 20, 1378-1386.