Journal
LUNG CANCER
Volume 63, Issue 2, Pages 201-209Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.lungcan.2008.05.005
Keywords
FCCP; ROS; Calu-6; ROS scavenger; GSH; Mitochondria
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Funding
- Korean Science and Engineering Foundation [R01-2006-000-10544-0]
- Korea Research Foundation
- Government of the Republic of Korea (MOEHRD)
- National Research Foundation of Korea [R01-2006-000-10554-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone (FCCP) is an uncoupler of mitochondrial oxidative phosphorylation in eukaryotic cells. Here, we investigated an involvement of O-2(center dot-) and GSH in FCCP-induced Calu-6 cell death and examined whether ROS scavengers rescue cells from FCCP-induced cell death. Levels of intracellular O-2(center dot-) were markedly increased depending on the concentrations (5-100 mu M) of FCCP. A depletion of intracellular GSH content was also observed after exposing cells to FCCP Stable SOD mimetics, Tempol and Tiron did not change the levels of intracellular O-2(center dot-), apoptosis and the loss of mitochondrial membrane potential (DIP m). Treatment with thiol antioxidants, NAC and DTT, showed the recovery of GSH depletion and the reduction of O-2(center dot-) levels in FCCP-treated cells, which were accompanied by the inhibition of apoptosis. In contrast, BSO, a well-known inhibitor of GSH synthesis, aggravated GSH depletion, oxidative stress of O-2(center dot-) and cell death in FCCP-treated cells. Taken together, our data suggested that FCCP as an O-2(center dot-) generator, induces apoptosis via the depletion of intracellular GSH contents in Calu-6 cells. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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