A bioluminescent enzyme immunoassay for prostaglandin E2 using Cypridina luciferase

Prostaglandin E-2 is one of the major cyclooxygenase metabolites of arachidonic acid. We developed a competitive immunosorbent assay for prostaglandin E-2 utilizing a bioluminescent enzyme Cypridina luciferase. The prostaglandin E-2 amount could be quantified over the concentration ranging from 7.8 to 500 pg/mL. The amount of unlabeled prostaglandin E-2 required to displace 50% of the maximal binding of Cypridina luciferase-labeled prostaglandin E-2 (B/B-0) was approximately 35 pg/mL. The results show a great potential of Cypridina luciferase as a new labeling enzyme for enzyme-linked immunosorbent assay. Copyright (C) 2009 John Wiley & Sons, Ltd.