Journal
LIPIDS
Volume 49, Issue 4, Pages 327-333Publisher
WILEY
DOI: 10.1007/s11745-014-3886-7
Keywords
Crambe; PDAT; DGAT; Microsomal preparation; Triacylglycerol; Erucic acid; Lipids
Funding
- European commission FP7 project ICON
- Vinnova
- VR
- FORMAS
- BBSRC [BB/H004459/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/H004459/1] Funding Source: researchfish
Ask authors/readers for more resources
The triacylglycerol of Crambe abyssinica seeds consist of 95 % very long chain (> 18 carbon) fatty acids (86 % erucic acid; 22:1(a dagger 13)) in the sn-1 and sn-3 positions. This would suggest that C. abyssinica triacylglycerols are not formed by the action of the phospholipid:diacylglycerol acyltransferase (PDAT), but are rather the results of acyl-CoA:diacylglycerol acyltransferase (DGAT) activity. However, measurements of PDAT and DGAT activities in microsomal membranes showed that C. abyssinica has significant PDAT activity, corresponding to about 10 % of the DGAT activity during periods of rapid seed oil accumulation. The specific activity of DGAT for erucoyl-CoA had doubled at 19 days after flowering compared to earlier developmental stages, and was, at that stage, the preferred acyl donor, whereas the activities for 16:0-CoA and 18:1-CoA remained constant. This indicates that an expression of an isoform of DGAT with high specificity for erucoyl-CoA is induced at the onset of rapid erucic acid and oil accumulation in the C. abyssinica seeds. Analysis of the composition of the acyl-CoA pool during different stages of seed development showed that the percentage of erucoyl groups in acyl-CoA was much higher than in complex lipids at all stages of seed development except in the desiccation phase. These results are in accordance with published results showing that the rate limiting step in erucic acid accumulation in C. abyssinica oil is the utilization of erucoyl-CoA by the acyltransferases in the glycerol-3-phosphate pathway.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available