Journal
LIPIDS
Volume 44, Issue 1, Pages 77-83Publisher
WILEY
DOI: 10.1007/s11745-008-3248-4
Keywords
Glyceroglycolipid; Sulfoquinovosyldiacylglycerol; Monogalactosyldiacylglycerol; Digalactosyldiacylglycerol; Acidic lipid; Plant; Chloroplast; ELSD; Binary gradient; HPLC
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Membrane lipids of photosynthetic organisms consist of glycerophospholipids and glyceroglycolipids. We investigated a method for the simultaneous quantitative analysis of neutral and acidic lipids using HPLC-ELSD, and quantified monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and sulfoquinovosyldiacylglycerol (SQDG). Ten complex lipid classes were separated with a binary gradient system consisting of chloroform and methanol-acetone-water-acetic acid (30:60:9:1, v/v/v/v) with 0.3% triethylamine (pH 4), and were eluted within 16 min. The contents of SQDG in ten edible plants ranged from 3 to 101 mg/100 g, and were positively correlated to the neutral glyceroglycolipids contents.
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