Journal
LIFE SCIENCES
Volume 85, Issue 1-2, Pages 60-69Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2009.04.017
Keywords
TRPA1; Miogadial; Miogatrial; Polygodial; TRPV1; HC-030031
Funding
- Kunitoshi Uchida of National Institutes of Natural Sciences
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Aims: Most of the terpenoids with an alpha,beta-unsaturated 1,4-dialdehyde moiety, which are found in plants, fungi, and insects, have a pungent taste. However, the neural receptors responsible for the pungency of these terpenoids have not been identified yet. The transient receptor potential ankyrin 1 (TRPA1) and transient receptor potential vanilloid 1 (TRPV1), which are expressed in the nociceptive neurons, induce a sensation of heat on activation by some pungent ingredients in food. In this study, we selected miogadial (MD), miogatrial (MT), and polygodial (PG) from the terpenoids with an alpha,beta-unsaturated 1,4-dialdehyde moiety and examined the effects of these 3 terpenoids on TRPA1 or TRPV1. Main methods: TRPV1 and TRPA1 activity by 3 terpenoids were evaluated using Ca2+ imaging and patch-clamp methods in mammalian cells that express TRP heterologously and mouse sensory neurons. Key findings: The 3 terpenoids activated TRPA1 that was heterologously expressed in HEK293 or CHO cells. The potencies of activation by the 3 terpenoids were equal and almost 10 times stronger than that of allyl isothiocyanate (AITC), which is known as the most potent TRPA1 agonist among all natural products. Moreover, these 3 terpenoids exhibited increased intracellular Ca2+ concentration in mouse sensory neuron cells compared to AITC. High concentrations of the 3 terpenoids also activated TRPV1 that was heterologously expressed in HEK293 cells. Significance: These results indicated that MD, MT, and PC were more potent in activating TRPA1 than TRPV1, and suggested that they primarily activate TRPA1 to induce pungency. (C) 2009 Elsevier Inc. All rights reserved.
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