Journal
LIFE SCIENCES
Volume 83, Issue 7-8, Pages 236-241Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2008.06.011
Keywords
hyperbaric oxygen preconditioning; hypoxia inducible factor-1; vascular endothelial growth factor
Funding
- National Natural Science Foundation of China [30500579]
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Hyperbaric oxygen preconditioning (HBO-PC) increases the level of HIF-1 alpha (hypoxia inducible factor-lot) and its target gene VEGF(vascular endothelial growth factor) which is involved in angiogenesis. Liver regeneration is an angiogenesis-dependent process. We hypothesized that HIF-1a and VEGF mediated the angiogenesis effect of HBO-PC on regenerating rat liver. Male Sprague Dawley rats received HBO-PC followed by 70% partial hepatectomy. Proliferation of hepatocytes and endothelial cells was evaluated by BrdU (bromodeoxyuridine) staining. Microvascular density was assessed by immunohistochemistry. mRNA expression of HIF-1 alpha was assessed by quantitative RT-PCR and protein levels of HIF-1 alpha and VEGF were assessed by western blot. HIF-1 alpha DNA-binding activity was determined with an ELISA-based kit. HBO-PC increased the proliferation index of endothelial cells and microvascular density at 48 h after partial hepatectomy. The protein level and DNA-binding activity of HIF-1 alpha and the protein level of VEGF were increased by HBO-PC before and after partial hepatectomy. Partial hepatectomy alone also increased proliferation index and the expressions of HIF-1 alpha and VEGF. Our results indicated that the angiogenesis effect of HBO-PC on liver after partial hepatectomy could be achieved by increased HIF-1 alpha activity and VEGF expression. However, the angiogenic effect of HBO-PC is moderate and HBO-PC failed to produce additional effect on the enhancement of HIF-1 alpha and VEGF induced by partial hepatectomy alone. (C) 2008 Elsevier Inc. All rights reserved.
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