Journal
LEUKEMIA RESEARCH
Volume 33, Issue 2, Pages 340-343Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.leukres.2008.07.005
Keywords
Myelodysplastic syndrome; Refractory anemia; Refractory anemia with ring sideroblasts; Oxidative DNA damage; Comet assay
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Funding
- Ministry of Health of the Czech Republic [NR8265-3/2005, VZMSM21620808, AVOZ50390512]
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Bone marrow aspirates of 19 patients with low-risk myelodysplastic syndromes (MDS) and 14 control subjects were collected ill order to assess the level of oxidative DNA damage. Glycophorin A positive and negative cells separated by miniMACS magnetic cell sorting were Subjected to single cell gel electrophoresis (comet assay) combined with enzymes of base excision repair (endonuclease III and formamido-pyrimidine glycosylase) that specifically recognize oxidized nucleotides. Compared to controls, MDS patients exhibited a significant increase of oxidative damage to DNA which could contribute to genomic instability and disease progression. (C) 2008 Elsevier Ltd. All rights reserved.
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