Journal
LEUKEMIA RESEARCH
Volume 32, Issue 2, Pages 335-338Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.leukres.2007.05.007
Keywords
lymphoma; molecular diagnosis; PCR; clonality; immunoglobulin heavy chain gene
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A biopsy of a nasal mass that had morphologic and immunostaining features consistent with a B-cell lymphoma was studied for clonality using PCR of the IgH gene. An unexpectedly low molecular weight DNA fragment of approximately 140 bp (acceptable size limit: 250-295 bp) was obtained using FR2 and JH primers. The sequence of this DNA was consistent with a clonal IgH rearrangement followed by a deletion that removed most of the downstream portion of the V segment. Thus, the biopsy contained a monoclonal population of B-lymphocytes, consistent with a diagnosis of lymphoma. This work illustrates that bands outside of the size range expected from PCR of the antigen receptor genes may still be consistent with a monoclonal result. (C) 2007 Elsevier Ltd. All rights reserved.
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