Journal
LEUKEMIA
Volume 26, Issue 9, Pages 1908-1975Publisher
SPRINGERNATURE
DOI: 10.1038/leu.2012.120
Keywords
EuroFlow; antibody panel; lymphoma; flow cytometry; 8-color immunostaining; standardization; hematological malignancies
Categories
Funding
- European Commission [STREP EU-FP6, LSHB-CT-2006-018708]
- Spanish Network of Cancer Research Centers [ISCIII RTICC-RD06/0020/0035-FEDER]
- Fondo de Investigacion Sanitaria, Ministerio de Ciencia e Innovacion (Madrid, Spain) [FIS 08/90881, FIS PS09/02430]
- Consejeria de Educacion, Junta de Castilla y Leon (Valladolid, Spain) [GR37 EDU/894/2009, SA016-A-09]
- Direccion General de Cooperacion Internacional y Relaciones Institucionales, Secretaria de Estado de Investigacion, Ministerio de Ciencia e Innovacion (Madrid, Spain) [PIB2010BZ-00565]
- Ministry of Health Czech Republic [NT/12425-4, NS/10480-3]
- Charles University [23010]
- UNCE [204012]
- International Society for Advancement of Cytometry (ISAC)
- Leukemia and Lymphoma Research (LLR)
Ask authors/readers for more resources
Most consensus leukemia & lymphoma antibody panels consist of lists of markers based on expert opinions, but they have not been validated. Here we present the validated EuroFlow 8-color antibody panels for immunophenotyping of hematological malignancies. The single-tube screening panels and multi-tube classification panels fit into the EuroFlow diagnostic algorithm with entries defined by clinical and laboratory parameters. The panels were constructed in 2-7 sequential design-evaluation-redesign rounds, using novel Infinicyt software tools for multivariate data analysis. Two groups of markers are combined in each 8-color tube: (i) backbone markers to identify distinct cell populations in a sample, and (ii) markers for characterization of specific cell populations. In multi-tube panels, the backbone markers were optimally placed at the same fluorochrome position in every tube, to provide identical multidimensional localization of the target cell population(s). The characterization markers were positioned according to the diagnostic utility of the combined markers. Each proposed antibody combination was tested against reference databases of normal and malignant cells from healthy subjects and WHO-based disease entities, respectively. The EuroFlow studies resulted in validated and flexible 8-color antibody panels for multidimensional identification and characterization of normal and aberrant cells, optimally suited for immunophenotypic screening and classification of hematological malignancies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available