Journal
LEUKEMIA
Volume 27, Issue 2, Pages 353-361Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/leu.2012.208
Keywords
acute myeloid leukemia; nnicroRNA; p53
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Funding
- Bundesministerium fur Bildung und Forschung (NGFNplus Grant) [01GS0871]
- Deutsche Jose Carreras Stiftung e.V. [DJCLS R 06/41v]
- Deutsche Forschungsgemeinschaft [Heisenberg-Stipendium BU 1339/3-1]
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Recently, the p53-miR-34a network has been identified to have an important role in tumorigenesis. As in acute myeloid leukemia with complex karyotype (CK-AML) TP53 alterations are the most common known molecular lesion, we further analyzed the p53-nniR-34a axis in a large cohort of CK-AML with known TP53 status (TP53(altere)d, n = 57; Tp53(unaltered), n =31; altered indicates loss and/or mutation of TP53). Profiling microRNA (miRNA) expression delineated TP53 alteration-associated miRNA profiles, and identified miR-34a and nniR-100 as the most significantly down- and upregulated miRNA, respectively. Moreover, we found a distinct miR-34a expression-linked gene expression profile enriched for genes belonging to p53-associated pathways, and implicated in cell cycle progression or apoptosis. Clinically, low miR-34a expression and TP53 alterations predicted for chemotherapy resistance and inferior outcome. Notably, in TP53(unaltered) CK-AML, high miR-34a expression predicted for inferior overall survival (OS), whereas in TP53(biallelic altered) CK-AML, high miR-34a expression pointed to better OS. Thus, detailed molecular profiling links impaired p53 to decreased miR-34a expression, but also identifies p53-independent miR-34a induction mechanisms as shown in TP53(biallelic altered) cell lines treated with 15-deoxy-Delta(12,14)-prostaglandin. An improved understanding of this mechanism might provide novel therapeutic options to restore miR-34a function and thereby induce cell cycle arrest and apoptosis in Tp53(altered) CK-AML. Leukemia (2013) 27, 353-361; doi:10.1038/leu.2012.208
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