Journal
RADIATION ONCOLOGY
Volume 10, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/s13014-015-0350-7
Keywords
Micro-irradiation; Particles; Live-cell imaging; Fluorescence microscopy
Funding
- DFG-Cluster of Excellence 'Munich Advanced Photonics'
- EU FP7 DoReMi Network of Excellence
- German Federal Ministry for the Environment, Nature Conservation and Nuclear Safety (BMU) [3610S30015, 3610S30016]
- Maier Leibnitz Laboratory Munich
Ask authors/readers for more resources
Ion microbeams are important tools in radiobiological research. Still, the worldwide number of ion microbeam facilities where biological experiments can be performed is limited. Even fewer facilities combine ion microirradiation with live-cell imaging to allow microscopic observation of cellular response reactions starting very fast after irradiation and continuing for many hours. At SNAKE, the ion microbeam facility at the Munich 14 MV tandem accelerator, a large variety of biological experiments are performed on a regular basis. Here, recent developments and ongoing research projects at the ion microbeam SNAKE are presented with specific emphasis on live-cell imaging experiments. An overview of the technical details of the setup is given, including examples of suitable biological samples. By ion beam focusing to submicrometer beam spot size and single ion detection it is possible to target subcellular structures with defined numbers of ions. Focusing of high numbers of ions to single spots allows studying the influence of high local damage density on recruitment of damage response proteins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available