Journal
LETTERS IN APPLIED MICROBIOLOGY
Volume 57, Issue 6, Pages 476-483Publisher
WILEY
DOI: 10.1111/lam.12138
Keywords
Brazil; capsular serotype; fish; MLST; virulence genes
Categories
Funding
- Furnas Centrais Eletricas
- Nacional Institute of Science and Technology-INCT/CNPq/UFMG [573899/2008-8]
- Ministry of Fisheries and Aquaculture (MPA), Brazil
- CAPES
- FAPEMIG
- CNPq
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This study aimed to assess the genetic diversity of fish isolates of Streptococcus agalactiae by capsular serotyping, MLST and the pattern of selected virulence genes. Forty-six isolates from Nile tilapia and Amazon catfish were screened by PCR for the twelve virulence genes. The molecular capsular type and sequence type (ST) were determined. Two capsular types (Ia and Ib) and four STs (103, 260, 552 and 553) were identified. The ST-552 and ST-553 represent new allelic combinations. Variable results were found for the genes gbs2018-6, lmb, hylB and cylE. The combined evaluation of serotype, sequence type and pattern of the presence or absence of cylE and hylB allowed the classification of isolates into nine genetic profiles (I-IX). The proposed scheme showed higher discriminatory power and was able to detect evolutionary events missed by MLST analysis. This study provides new information about the genetic diversity of fish pathogenic Strep.agalactiae, and the proposed scheme was shown to be an improved approach to genotyping these strains.
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