4.3 Article

Characterization of a Campylobacter fetus-like strain isolated from the faeces of a sick leopard tortoise (Stigmochelys pardalis) using matrix-assisted laser desorption/ionization time of flight as an alternative to bacterial 16S rDNA phylogeny

Journal

LETTERS IN APPLIED MICROBIOLOGY
Volume 58, Issue 4, Pages 338-343

Publisher

WILEY-BLACKWELL
DOI: 10.1111/lam.12194

Keywords

new species; molecular typing; characterization; leopard tortoise; Campylobacter

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This article describes the isolation and characterization of a Campylobacter-like isolate originating from the faeces of a sick leopard tortoise. Molecular as well as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) characterization suggests that it could correspond to a new Campylobacter species. Significance and Impact of Study The major impact of this work is the demonstration that proteomics and especially MALDI-TOF typing can be used as an alternative method to 16S rDNA sequencing for phylogeny and can lead to the discovery of new Campylobacters.

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