Journal
LETTERS IN APPLIED MICROBIOLOGY
Volume 55, Issue 4, Pages 290-294Publisher
WILEY
DOI: 10.1111/j.1472-765X.2012.03293.x
Keywords
Aeromonas spp; ColE2-type; qnrS2 gene; quinolone resistance
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Funding
- Korean Research Foundation [KRF-2008-331-E00385]
- National Research Foundation of Korea [2008-331-E00385] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Aims: Isolation and full sequence analysis of ColE-type plasmid, which carries the qnrS2 gene. Methods and Results: Quinolone resistance (qnrS2) gene-carrying plasmids were isolated from Aeromonas sobria and Aeromonas hydrophila strains, and plasmid sequencing was achieved by a primer-walking approach. The total sizes of these plasmids (pAQ2-1 and pAQ2-2) were 6900 bp and 6903 bp, respectively, and they were 99.1% identical to each other. The genes (oriV and repA) for plasmid replication were organized similar to the corresponding genes in the ColE2-type plasmids, pAsa3 and pAsa1, isolated from Aeromonas salmonicida subsp. salmonicida, but the gene (mobA) for mobilization was homologue to ColE1-type plasmid (pAsa2) from Aer. salmonicida subsp. salmonicida. Additionally, the qnrS2 gene was part of a mobile insertion cassette element in the plasmid. Conclusions: Two plasmids were assumed to be the same plasmid, and this identification of a plasmid-mediated qnrS2 gene from the two different strains underlines a possible diffusion of these resistance determinants in an aquaculture system. Significance and Impact of the Study: This is the first finding of the ColE-type plasmid carrying the qnrS2 gene.
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