Journal
LETTERS IN APPLIED MICROBIOLOGY
Volume 49, Issue 6, Pages 791-795Publisher
WILEY
DOI: 10.1111/j.1472-765X.2009.02744.x
Keywords
antiviral; bovine herpesvirus; chlorophyllin; plaque; poliovirus
Categories
Funding
- CNPq
- CAPES
- UEL
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Aims: Chlorophyllin (CHLN), a synthetic derivative of chlorophyll, was assayed in the replication of poliovirus (PV-1) and bovine herpesvirus (BoHV-1) in HEp-2 cell cultures. Methods and Results: Virucidal activity of CHLN was evaluated and the time-of-addition assay was performed as follows: before the infection (-1 and -2 h), at the time of the infection (0 h) and after the infection (1 and 2 h). Plaque reduction assay (PRA) showed that CHLN inhibited BoHV-1 and PV-1 infection and the 50% inhibitory concentrations (IC50) against BoHV-1 and PV-1 infection were 8 center dot 6 and 19 center dot 8 mu g ml-1, respectively. The time-of-addition study demonstrated that the CHLN was effective inhibiting viral replication in 51% and 66 center dot 5% for PV-1 and BoHV-1, respectively, at the highest concentration of 20 center dot 0 mu g ml-1, when added during the infection. The directed effect of CHLN on viral strains demonstrated an inhibition of 62% and 66 center dot 4% for PV-1 and BoHV-1, respectively, by PRA. Conclusions: These results demonstrated that CHLN could be used as an antiviral suggesting directed activity on virus particles and on virus-receptor sites to BoHV. For poliovirus, CHLN also demonstrated virucide activity, moreover, showed to inhibit early steps of the replication cycle. Significance and Impact of the Study: CHLN demonstrated promising selectivity index for both virus strains; therefore, it can be used for the development of an antiviral agent.
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