Journal
LETTERS IN APPLIED MICROBIOLOGY
Volume 47, Issue 5, Pages 393-398Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1472-765X.2008.02443.x
Keywords
BOX-PCR; enterobacterial repetitive intergenic consensus-PCR; pulsed-field gel electrophoresis; repetitive extragenic palindromic-PCR; Stenotrophomonas maltophilia
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Funding
- China Medical University [CMU95-152, CMU95-102]
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Aims: In this study, three facile repetitive-sequence PCR (rep-PCR) techniques have been compared with the pulsed-field gel electrophoresis (PFGE) method for differentiating the genetic relatedness of clinical Stenotrophomonas maltophilia isolates. Methods and Results: The dendrograms of 20 S. maltophilia isolates were constructed based on the data obtained from PFGE and three PCR-based methods, i.e. enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR), BOX-PCR and repetitive extragenic palindromic-PCR (REP-PCR). When compared with PFGE, ERIC-PCR displayed a much lower discriminatory power, whereas BOX-PCR and REP-PCR had a comparable discriminatory power for close genetic-related isolates. Conclusion: BOX-PCR and REP-PCR can be convenient and effective methods for evaluating the close genetic relatedness of clinical S. maltophilia isolates. Significance and Impact of the Study: A rapid method for determining S. maltophilia's close genetic relatedness provides a convenient tool for understanding the epidemiology of S. maltophilia.
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