Journal
LASERS IN MEDICAL SCIENCE
Volume 27, Issue 3, Pages 621-627Publisher
SPRINGER LONDON LTD
DOI: 10.1007/s10103-011-1006-y
Keywords
Laser diode; Lipopolysaccharide; Inflammatory marker; Osteosarcoma cell
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Funding
- National Science Council [NSC 99-2314-B-040-014-MY3]
- Taiwan Association of Orthodontists
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In this study, the aim is to investigate the cytologic effects of inflammatory bone cells after in vitro low-level laser therapy (LLLT). A human osteosarcoma cell line (MG63) was cultured, infected with lipopolysaccharide (LPS) and exposed to low-level laser treatment at 5 or 10 J/cm(2) using a 920 nm diode laser. MG63 cell attachment was observed under a microscope, and cell viability was quantified by mitochondrial colorimetric assay (MTT). LPS-treated MG63 cells were irradiated with LLLT, and the inflammatory markers iNOS, TNF-alpha and IL-1, were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The data were collected and analyzed by one-way analysis of variance (ANOVA); p < 0.05 indicated a statistically significant difference. Low-level laser treatment on MG63 cells increased their ability to attach and survive. After irradiation, the expression levels of iNOS, TNF-alpha and IL-1 in LPS-infected MG63 cells decreased over time (p < 0.05). Conclusions: low-level diode laser treatment increased the MG63 cell proliferative ability and decreased the expression of inflammatory mediators in MG63 cells.
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