Journal
LASERS IN MEDICAL SCIENCE
Volume 26, Issue 3, Pages 299-305Publisher
SPRINGER LONDON LTD
DOI: 10.1007/s10103-010-0771-3
Keywords
Laser-induced fluorescence; Dental caries; Cavitated; Noncavitated; Receiver operating characteristic curve; Fluorescence intensity ratios
Categories
Funding
- CESS [Plan-260]
- Research Council (RC) of CESS
- Director of CESS
- RC of CESS, Trivandrum
- SC Department, Government of Kerala
- DST
- CSIR, New Delhi
- RC of CESS
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The potential of laser-induced fluorescence (LIF) spectroscopy for the characterization of different stages of dental caries using 404-nm diode laser excitation was investigated. In vitro spectra from 16 sound, 10 noncavitated carious and 10 cavitated carious molar teeth were recorded on a miniature fibre-optic spectrometer. The areas under the receiver operating characteristics (ROC-AUC) were calculated and one-way analysis of variance (ANOVA) was performed. The LIF spectra of the carious teeth showed two peaks at 635 and 680 nm in addition to a broad band seen at 500 nm in sound teeth. The fluorescence intensity ratios, F500/F635 and F500/F680, in carious teeth were always lower than those in sound teeth. The ROC-AUC for discriminating between carious and sound teeth was 0.94, and for discriminating between noncavitated and cavitated carious teeth was 0.87. Statistically significant differences (p < 0.001) were seen between sound, noncavitated carious and cavitated carious teeth. The results showed that LIF spectroscopy has the potential to be useful for characterizing different stages of caries in a clinical setting.
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