Journal
LASERS IN MEDICAL SCIENCE
Volume 25, Issue 1, Pages 33-39Publisher
SPRINGER LONDON LTD
DOI: 10.1007/s10103-008-0636-1
Keywords
Stem cells; Low-level laser; Epidermal growth factor; Viability; Proliferation
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Funding
- National Laser Centre of South Africa
- National Research Foundation of South Africa
- Council for Scientific and Industrial Research of South Africa
- University of Johannesburg
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The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm(2) using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm(2) on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.
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