Journal
LARYNGOSCOPE
Volume 122, Issue 2, Pages 467-472Publisher
WILEY
DOI: 10.1002/lary.22398
Keywords
Bacteria; bacterial sinusitis; human microbiome; sinus culture; middle meatus; Level of Evidence: 2b
Funding
- NIH [HG005964]
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Objectives/Hypothesis: The aim of this study was to compare microbiological culture-based and culture-independent (16S rRNA gene sequencing) methodologies for pathogen identification in chronic rhinosinusitis (CRS) patients. We hypothesized that bacterial culture and DNA sequencing would yield largely concurrent results, although sequencing would detect greater bacterial diversity, and the sinonasal microbiomes of CRS patients would differ in composition and diversity compared with non-CRS controls.
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