Journal
LANGMUIR
Volume 35, Issue 5, Pages 1379-1390Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.langmuir.8b01597
Keywords
-
Funding
- United States Special Operations Command [W81XWH-16-C-0219]
- Combat Casualty Care Research Program (JPC-6) [W81XWH-17-2-0045]
- National Council for the Improvement of Higher Education - CAPES (the Science without Borders project)
Ask authors/readers for more resources
Motivated by the lack of adventitious protein adsorption on zwitterionic polymer brushes that promise low noise and hence high analytical sensitivity for surface-based immunoassays, we explored their use as a substrate for immunoassay fabrication by the inkjet printing of antibodies. We observed that a poly(sulfobetaine)methacrylate brush on glass is far too hydrophilic to enable the noncovalent immobilization of antibodies by inkjet printing. To circumvent this limitation, we developed a series of hybrid zwitterionic-cationic surface coatings with tunable surface wettability that are suitable for the inkjet printing of antibodies but also have low protein adsorption. We show that in a microarray format in which both the capture and detection antibodies are discretely printed as spots on these hybrid brushes, a point-of-care sandwich immunoassay can be carried out with an analytical sensitivity and dynamic range that is similar to or better than those of the same assay fabricated on a PEG-like brush. We also show that the hybrid polymer brushes do not bind anti-PEG antibodies that are ubiquitous in human blood, which can be a problem with immunoassays fabricated on PEG-like coatings.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available