Journal
LANGMUIR
Volume 30, Issue 20, Pages 5695-5699Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la404146g
Keywords
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Funding
- Armenise-Harvard foundation
- CIBIO
- NSF [MCB-1244180]
- NIH [R01 GM078352]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1244180] Funding Source: National Science Foundation
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The construction of genetically encoded cellular mimics in compartments containing organized synthetic cytosols is desirable for the development of artificial cells. Phase separated aqueous domains were placed within water-in-oil emulsion droplets in a manner compatible with transcription and translation machinery. Aqueous two-phase and three-phase systems (ATPS and A3PS) were assembled with dextran, poly(ethylene glycol), and Ficoll. Aqueous two-phase systems were capable of supporting the cell-free expression of protein within water droplets, whereas the aqueous three-phase-based system did not give rise to detectable protein synthesis. The expressed protein preferentially partitioned to the dextran-enriched phase. The system could serve as a foundation for building cellular mimics with liquid organelles.
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