Journal
LANGMUIR
Volume 30, Issue 12, Pages 3432-3440Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la500464v
Keywords
-
Funding
- EU through the European regional development fund (ERDF)
- MINECO [MAT-2010-15982, MAT2010-15310, PRI-PIBAR-2011-1403, MAT2012-38043]
- JCyL [VA049A11, VA152A12, VA155A12]
- CIBER-BBN
- JCyL
- Instituto de Salud Carlos III under the Network Center of Regenerative Medicine and Cellular Therapy of Castilla and Leon
Ask authors/readers for more resources
Recent advances in genetic engineering now allow the synthesis of protein-based block corecombinamers derived from elastin-like peptide sequences with complete control of chemistry and molecular weight, thereby resulting in unique physical and biological properties. The individual blocks of the elastin-like block corecombinamers (ELbcR's) display different phase behaviors in aqueous solution, which leads to the thermally triggered self-assembly of nano-objects ranging from micelles to vesicles. Herein, the interaction of cationic surfactant dodecyl trimethylammonium bromide (DTAB), anionic surfactant dodecyl sodium sulfate (SDS), and nonionic surfactant octyl-beta-glucopyranoside (OG) with an ELbcR has been investigated by dynamic light scattering (DLS), the zeta potential and cryo-transmission electron microscopy (cryo-TEM). At 65 degrees C and neutral pH in aqueous solution, the ELbcR (E50A40) is associated into micelles with a diameter of 150 nm comprising a hydrophobic (A) core and a hydrophilic (E) anionic (from the glutamic acid residues) corona. The size of these self-assemblies can be controlled by adjusting the cosurfactant concentrations. Although the effects of surfactants on the self-assembly behavior of ELbcR's depend on the hydrocarbon chain length and headgroup of the surfactants, a general tendency to increase in size, which in some cases leads to flocculation and a phase-separated state, is observed. These results support the use of surfactants as a highly interesting means of controlling the self-assembly of ELbcR's in aqueous solution as well as their use in drug delivery and purification processes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available