Journal
LANGMUIR
Volume 29, Issue 39, Pages 12317-12327Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la402139g
Keywords
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Funding
- Chulalongkorn University Centenary Academic Development Project (Under the Center of Innovative Nanotechnology, Chulalongkorn University)
- National Research University Project of Thailand, Office of the Higher Education Commission [AM1006A]
- Thailand Research Fund [DBG5580003]
- Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University [RES560530126-AM]
- Thai Government Stimulus Package 2, under the Project for Establishment of Comprehensive Center for Innovative Food, Health Products and Agriculture [TKK2555]
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Gold nanoparticles stabilized by thermoresponsive polymer, poly(N-isopropylacrylamide) (PNIPAM-AuNPs) were prepared by surface grafting of thiol-terminated PNIPAM onto citrate-stabilized AuNPs. The color change of the PNIPAM-AuNPs solution from red to blue-purple without precipitation when the solution was heated to 40 degrees C, above the lower critical solution temperature (LCST) of PNIPAM, indicated the thermoresponsive property of the synthesized AuNPs. PNIPAM-AuNPs were used to detect proteins by chemical nose approach based on fluorescence quenching of fluorophore by AuNPs. An array-based sensing platform for.detection of six proteins, namely bovine serum albumin, lysozyme, fibrinogen, concanavalin A, hemoglobin, holo-transferrin human can be successfully developed from the PNIPAM-AuNPs having different molecular weights (4 and 8 kDa) and conformation (varied heat treatment from 25 to 40 degrees C) in combination with a tricationic branched phenylene-ethynylene fluorophore. From principal component analysis (PCA) followed by linear discriminant analysis (LDA), 100% accuracy of protein classification using a leave-one-out (LOO) approach can be achieved by using only two types of PNIPAM-AuNPs.
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