Journal
LANGMUIR
Volume 29, Issue 30, Pages 9457-9463Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la401068n
Keywords
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Funding
- A23685027
- A24760622
- JSPS
- [20106003]
- Grants-in-Aid for Scientific Research [24655157, 24510317, 13J05206, 23685027] Funding Source: KAKEN
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The availability of metal mesh device sensors has been investigated using surface-modified nickel mesh. Biotin was immobilized on the sensor surfaces consisting of silicon and nickel via a thiol-ene click reaction, known as the Michael addition reaction. Biotinylation on the maleimidated surface was confirmed by X-ray photoelectron spectroscopy. The binding of streptavidin to the biotinylated surfaces was evaluated using a quartz crystal microbalance and a metal mesh device sensor, with both techniques providing similar binding constant value. The recognition ability of the biotin immobilized using the thiol-maleimide method for streptavidin was comparable to that of biotin immobilized via several other methods. The adsorption of a biotin conjugate onto the streptavidin-immobilized surface via the biotin-streptvidin-biotin sandwich method was evaluated using a fluorescent microarray, with the results demonstrating that the biological activity of the streptavidin remained.
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