Journal
LANGMUIR
Volume 28, Issue 21, Pages 8120-8126Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la204863s
Keywords
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Funding
- Japan Society for the Promotion of Science (JSPS)
- Office of Science, Office of Basic Energy Science, of the U.S. Department of Energy [DE-AC02-05CH11231]
- Jane Coffin Childs Memorial Research Fund
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Previous reports have shown that synthetic DNA strands can be attached to the plasma membrane of living cells to equip them with artificial adhesion receptors that bind to complementary strands extending from material surfaces. This approach is compatible with a wide range of cell types, offers excellent capture efficiency, and can potentially be used to create complex multicellular arrangements through the use of multiple capture sequences. In this work, we apply an aluminum lift off lithography method to allow the efficient generation of complex patterns comprising different DNA sequences. The resulting surfaces are then demonstrated to be able to capture up to three distinct types of living cells in specific locations. The utility of this approach is demonstrated through the observation of patterned cells as they communicate by diffusion-based paracrine signaling. It is anticipated that the ability of this technique to create virtually any type of 2D heterogeneous cell pattern should prove highly useful for the examination of key questions in cell signaling, including stem cell differentiation and cancer metastasis.
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