Journal
LANGMUIR
Volume 27, Issue 20, Pages 12434-12442Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la202444s
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Funding
- West Virginia State Challenge [0304]
- ARO [G: W911NF-05-1-0309, P00002]
- National Science Foundation [CHE-0948293]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0948293] Funding Source: National Science Foundation
- EPSCoR
- Office Of The Director [0918949] Funding Source: National Science Foundation
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The synthesis of multithiolated DNA molecules that can be used to produce self-assembled monolayers of single-stranded DNA oligonucleotides on gold substrates is described. Generation 3 polyamidoamine (PAMAM) dendrimers were conjugated to DNA oligomers and functionalized with similar to 30 protected thiol groups. The protected thiol groups-thioacetate groups-allowed the dendrirner-DNA constructs to be stored in a buffer solution for at least 2 months before deprotection without any observable decrease in their ability to assemble into functional layers. The monolayers formed using these multithiolated DNA probe strands demonstrate target capture efficiencies comparable to those of analogous monolayers assembled with DNA functionalized with single thiol groups. A functional advantage of using dendrimer headgroups is the resistance to probe strand loss in prolonged exposure to buffer solutions at a high temperature (95 degrees C).
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