Journal
LANGMUIR
Volume 27, Issue 24, Pages 15345-15351Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la203382k
Keywords
-
Funding
- Pennsylvania Department of Health
- Pittsburgh Tissue Engineering Initiative (SAP) [4100056872]
- Ethel Charitable Trust
- DOE
Ask authors/readers for more resources
Cell separation technology is a key tool for biological studies and medical diagnostics that relies primarily on chemical labeling to identify particular phenotypes. An emergent method of sorting cells based on differential rolling on chemically patterned substrates holds potential benefits over existing technologies, but the underlying mechanisms being exploited are not well characterized. In order to better understand cell rolling on complex surfaces, a microfluidic device with chemically patterned stripes of the cell adhesion molecule P-selectin was designed. The behavior of HL-60 cells rolling under flow was analyzed using a high-resolution visual tracking system. This behavior was then correlated to a number of established predictive models. The combination of computational modeling and widely available fabrication techniques described herein represents a crucial step toward the successful development of continuous, label-free methods of cell separation based on rolling adhesion.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available