Journal
LANGMUIR
Volume 27, Issue 4, Pages 1387-1390Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la104346k
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Funding
- ERC
- Walters-Kundert Foundation
- EPSRC [EP/F035535/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/F035535/1] Funding Source: researchfish
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We demonstrate a supramolecular peptide separation approach by the selective immobilization of peptides bearing an N-terminal tryptophan onto a CB[8]-modified gold substrate, followed by electrochemical release. The CB[8]stabilized heteroternary complexes were characterized by H-1 NMR, ESI-MS, UV/vis, and fluorescence spectroscopy and cyclic voltammetry. Micropatterned CB[8]-modified gold substrates were found to trap only the recognizable N-tryptophan-containing peptides from a peptide mixture that could be visualized as green peptide arrays under fluorescence microscopy. Subsequently, the bound peptides were released from the modified substrates by the controlled single-electron reduction of viologen. The fully reversible trap-and-release process was repeated for 13 cycles, and the cumulative release profit 2 of the dye peptide conjugate was monitored by fluorescence spectroscopy, indicating that no degradation occurred.
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