Journal
LANGMUIR
Volume 27, Issue 8, Pages 4906-4913Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la200061h
Keywords
-
Funding
- NSF [CBET-0952624]
- 3M Non-Tenured Faculty Award
- University of Washington
- NOAA Oceans and Human Health Initiative
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0952624] Funding Source: National Science Foundation
Ask authors/readers for more resources
The development of nonfouling biomaterials to prevent nonspecific protein adsorption and cell/bacterial adhesion is critical for many biomedical applications, such as antithrombogenic implants and biosensors. In this work, we polymerize two types of hydroxy-functional methacrylates monomers of 2-hydroxyethyl methacrylate (HEMA) and hydroxypropyl methacrylate (HPMA) into polymer brushes on the gold substrate via surface-initiated atom transfer radical polymerization (SI-ATRP). We systematically examine the effect of the film thickness of polyHEMA and polyHPMA brushes on their antifouling performance in a wide range of biological media including single-protein solution, both diluted and undiluted human blood serum and plasma, and bacteria culture. Surface plasmon resonance (SPR) results show a strong correlation between antifouling property and film thickness. Too thin or too thick polymer brushes lead to large protein adsorption. Surfaces with the appropriate film thickness of similar to 25-45 rim for polyHPMA and similar to 20-45 nm for polyHEMA can achieve almost zero protein adsorption (< 0.3 ng/cm(2)) from single-protein solution and diluted human blood plasma and serum. For undiluted human blood serum and plasma, polyHEMA brushes at a film thickness of similar to 20-30 nm adsorb only similar to 3.0 and similar to 3.5 ng/cm(2) proteins, respectively, while polyHPMA brushes at a film thickness of similar to 30 nm adsorb more proteins of similar to-13.5 and similar to 50.0 ng/cm(2), respectively. Moreover, both polyHEMA and polyHPMA brushes with optimal film thickness exhibit very low bacteria adhesion. The excellent antifouling ability and long-term stability of polyHEMA and polyHPMA brushes make them, especially for polyHEMA, effective and stable antifouling materials for usage in blood-contacting devices.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available