Journal
LANGMUIR
Volume 26, Issue 4, Pages 2645-2649Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la902827y
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This paper describes a method to produce monodisperse cell-encapsulating microgel beads composed of a self-assembling peptide gel for three-dimensional (3D) cell culture. We used a 3D microfluidic axisymmetric now-focusing device with ail external gelation method. The finely powdered salts were dispersed into a continuous phase, and the Salts induced the gelation when in contact with the peptide solution. Over 93% of the cells survived after the encapsulation, and (he cells migrated and grew Within the gels. Applications of Our cell-encapsulating beads include bead-based cell assays in drug testing and engineering tissue constructs.
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