Journal
LANGMUIR
Volume 26, Issue 12, Pages 10392-10396Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la100227s
Keywords
-
Funding
- Office of Naval Research
- National Institutes of Health
- Institute for Collaborative Biotechnologies through the U.S. Army Research Office
Ask authors/readers for more resources
We report a single-step, single-reagent, label-free, isothermal electrochemical DNA sensor based on the phenomenon of target recycling. The sensor exploits strand-specific exonuclease activity to achieve the selective enzymatic digestion of target/probe duplexes. This results in a permanent change in the probe structure that yields an increased faradaic current and liberates the intact target molecule to interact with additional detection probes to achieve further signal amplification. Using this architecture, we achieve an improved detection limit in comparison to hybridization-based sensors without amplification. We also demonstrate a 16-fold signal amplification factor at low target concentrations. Combined with the advantages of electrochemical detection and its ready integration with microelectronics, our approach may represent a promising path toward direct DNA detection at the point of care.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available