Journal
LANGMUIR
Volume 25, Issue 9, Pages 5120-5126Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la804140r
Keywords
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Funding
- NIH [R01HG002298, 5T32GM08349]
- NSF [CHE-0809095]
- MPS/CHE
- BIO/MCB Divisions
- University of Wisconsin Industrial and Economic Development Research
- GWC Technologies
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0809095] Funding Source: National Science Foundation
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Amorphous carbon thin films are easily deposited at room temperature, readily functionalized with alkene-containing molecules through a UV photochemical reaction, and provide a robust surface capable of supporting array fabrication. Relatively few attachment chemistries for the fabrication of small organic molecule and/or biomolecule arrays on carbon substrates have been described to date. Here, acyl chloride-terminated amorphous carbon substrates were fabricated, characterized, and used to attach alcohol-, thiol-, and amine-containing small molecules. Oligonucleotide arrays of thiol- and amine-modified oligonucleotides were also prepared on these substrates. The hybridization density, average fluorescence signal of hybridized features, and average background fluorescence of oligonucleotide arrays prepared on acyl chloride-modified substrates were compared to the same parameters for oligonucleotide arrays prepared on maleimide- and aldehyde-modified substrates.
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