Journal
LANGMUIR
Volume 25, Issue 2, Pages 1233-1237Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la803556p
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Funding
- Defense Advanced Research Project Agency [FA9550-06-C-00C]
- National Science Foundation [CHE-0616505]
- Japan Society for the Promotion of Science
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Translocation of single-stranded DNA through alpha-hemolysin (alpha-HL) channels is investigated in glycerol/water mixtures containing 1 M KCl. Experiments using glass nanopore membranes as the lipid bilayer support demonstrate that the translocation velocities of poly(deoxyadenylic acid), poly(deoxycytidylic acid), and poly(deoxythymidylic acid) 50-mers are decreased by a factor of similar to 20 in a 63/37 (vol %) glycerol/water mixture, relative to aqueous solutions. The ion conductance of alpha-HL and the entry rate of the polynucleotides into the protein channel also decrease with increasing viscosity. Precise control of translocation parameters by adjusting viscosity provides a potential means to improve sequencing methods based on ion channel recordings.
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