Surface-Supported Multilayers Decorated with Bio-active Material Aimed at Light-Triggered Drug Delivery

In this work, we report oil the functionalization of layer-by-layer films with gold nanoparticles, microcapsules, and DNA molecules by spontaneous incorporation into the film. Exponentially growing films from biopolymers, namely, hyaluronic acid (HA) and poly-L-lysine (PLL), and linearly growing films from the synthetic polymers, namely, poly(styrene sulfonate) (PSS) and poly(allylamine hydrochloride) (PAH), were examined for the embedding. The studied (PLL/HA)(24)/PLL and (PAH/PSS)(24)/PAH films are later named HA/PLL and PSS/PAH films, respectively. The HA/PLL film has been found to be more efficient for both particle and DNA embedding than PSS/PAH because of spontaneous PLL transport from the interior of the whole HA/PLL film to the surface in order to make additional contact with embedded particles or DNA. DNA and nanoparticles call be immobilized in HA/PLL films, reaching loading capacities of 1.5 and 100 mu g/cm(2), respectively. The capacities of PSS/PAH Films are 5 and 12 times lower than that for films made from biopolymers. Polyelectrolyte microcapsules adsorb irreversibly on the HA/PLL film surface as single particles whereas very poor interaction was observed for PSS/PAH. This intrinsic property of the HA/PLL film is due to the high mobility of PLL within the film whereas the structure of the PSS/PAH film is frozen in. Gold nanoparticles and DNA form micro meter-sized aggregates or patches on the HA/PLL Film surface. The diffusion of nanoparticles and DNA into the HA/PLL film is restricted at room temperature, but DNA diffusion is triggered by heating to 70 degrees C leading to homogeneous filling of the film with DNA. The film has not only a high loading capacity but also call be activated by biofriendly near-infrared (IR) laser light, thanks to the gold nanoparticle aggregates oil the film surface. Composite HA/PLL films with embedded gold nanoparticles and DNA call be activated by light, resulting ill DNA release. We assume that the mechanism of the release is dependent oil the disturbance ill bonding between doping PLL and DNA, which is induced by local thermal decomposition of the HA/PLL network in the film when the film is exposed to IR light. Remote IR-light activation of dextran-filled microcapsules modified by gold nanoparticles and integrated into the HA/PLL film is also demonstrated, revealing an alternative release pathway using immobilized light-sensitive carriers (microcapsules).